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  • 1.
    Ziels, Ryan M.
    et al.
    Department of Civil Engineering, The University of British Columbia, Vancouver, BC, Canada.
    Svensson, Bo H
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Sundberg, Carina
    Linköpings universitet, Institutionen för ekonomisk och industriell utveckling, Industriell miljöteknik. Linköpings universitet, Filosofiska fakulteten.
    Larsson, Madeleine
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Karlsson, Anna
    Scandinavian Biogas Fuels AB, Stockholm, Sweden.
    Shakeri Yekta, Sepehr
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Microbial rRNA gene expression and co-occurrence profiles associate with biokinetics and elemental composition in full-scale anaerobic digesters2018Ingår i: Microbial Biotechnology, ISSN 1751-7907, E-ISSN 1751-7915, Vol. 11, nr 4, s. 694-709Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This study examined whether the abundance and expression of microbial 16S rRNA genes were associated with elemental concentrations and substrate conversion biokinetics in 20 full-scale anaerobic digesters, including seven municipal sewage sludge (SS) digesters and 13 industrial codigesters. SS digester contents had higher methane production rates from acetate, propionate and phenyl acetate compared to industrial codigesters. SS digesters and industrial codigesters were distinctly clustered based on their elemental concentrations, with higher concentrations of NH3-N, Cl, K and Na observed in codigesters. Amplicon sequencing of 16S rRNA genes and reverse-transcribed 16S rRNA revealed divergent grouping of microbial communities between mesophilic SS digesters, mesophilic codigesters and thermophilic digesters. Higher intradigester distances between Archaea 16S rRNA and rRNA gene profiles were observed in mesophilic codigesters, which also had the lowest acetate utilization biokinetics. Constrained ordination showed that microbial rRNA and rRNA gene profiles were significantly associated with maximum methane production rates from acetate, propionate, oleate and phenyl acetate, as well as concentrations of NH3-N, Fe, S, Mo and Ni. A co-occurrence network of rRNA gene expression confirmed the three main clusters of anaerobic digester communities based on active populations. Syntrophic and methanogenic taxa were highly represented within the subnetworks, indicating that obligate energy-sharing partnerships play critical roles in stabilizing the digester microbiome. Overall, these results provide new evidence showing that different feed substrates associate with different micronutrient compositions in anaerobic digesters, which in turn may influence microbial abundance, activity and function.

  • 2.
    Moestedt, J.
    et al.
    Linköpings universitet, Biogas Research Center. Department of R&D Biogas, Tekniska verken i Linköping AB, Linköping, Sweden; Department of Microbiology, BioCenter, University of Agricultural Sciences, Uppsala, Sweden.
    Nordell, E.
    Linköpings universitet, Biogas Research Center. Department of R&D Biogas, Tekniska verken i Linköping AB, Linköping, Sweden.
    Shakeri Yekta, Sepehr
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Lundgren, J.
    Linköpings universitet, Biogas Research Center. Department of R&D Biogas, Tekniska verken i Linköping AB, Linköping, Sweden.
    Marti, M.
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Ejlertsson, Jörgen
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center. Scandinavian Biogas Fuels AB, Sweden.
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Björn, Annika
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste2016Ingår i: Waste Management, ISSN 0956-053X, E-ISSN 1879-2456, Vol. 47, nr Pt A, s. 11-20Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This study used semi-continuous laboratory scale biogas reactors to simulate the effects of trace-element addition in different combinations, while degrading the organic fraction of municipal solid waste and slaughterhouse waste. The results show that the combined addition of Fe, Co and Ni was superior to the addition of only Fe, Fe and Co or Fe and Ni. However, the addition of only Fe resulted in a more stable process than the combined addition of Fe and Co, perhaps indicating a too efficient acidogenesis and/or homoacetogenesis in relation to a Ni-deprived methanogenic population. The results were observed in terms of higher biogas production (+9%), biogas production rates (+35%) and reduced VFA concentration for combined addition compared to only Fe and Ni. The higher stability was supported by observations of differences in viscosity, intraday WA-and biogas kinetics as well as by the 16S rRNA gene and 16S rRNA of the methanogens.(c) 2015 Elsevier Ltd. All rights reserved.

  • 3.
    Moestedt, Jan
    et al.
    Linköpings universitet, Biogas Research Center. SLU.
    Nordell, Erik
    Linköpings universitet, Biogas Research Center. Tekniska Verken i Linköping.
    Lundgren, Jesper
    Linköpings universitet, Biogas Research Center.
    Genero Marti, Magali
    Linköpings universitet, Biogas Research Center.
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Ejlertsson, Jörgen
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Björn, Annika
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste2014Konferensbidrag (Refereegranskat)
  • 4.
    Bergfur, Jenny
    et al.
    Linköpings universitet, Institutionen för tema, Tema Miljöförändring. Linköpings universitet, Filosofiska fakulteten.
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Leaf-litter-associated fungi and bacteria along temporal and environmental gradients in boreal streams2014Ingår i: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 73, nr 3, s. 225-234Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Aquatic fungi and bacteria have long been recognized as key drivers in ecosystem processes such as leaf litter decomposition. We examined fungal and bacterial communities on decaying alder Alnus glutinosa (L.) Gaertner leaves along a gradient of increasing agricultural land use and associated nutrient enrichment in 9 boreal streams during 4 separate seasons (fall 2003, spring 2005, fall 2005, and spring 2006). Denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reactions (qPCR) showed that agricultural land use had significant effects on both bacterial and fungal communities, and on the ratios of fungi to total microbes associated with decomposing leaf litter. Furthermore, landscape factors and fluvial geomorphology appeared to influence the community composition of fungi and bacteria. Seasonal effects were found for fungal community structure only, indicating a higher temperature sensitivity of fungi compared to bacteria.

  • 5.
    Robroek, Bjorn J. M.
    et al.
    University of Utrecht, Netherlands.
    Jasper Wubs, E.R.
    Netherlands Institute of Ecology (NIOO-KNAW), Netherlands .
    Generó, Magali Marti
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Hälsouniversitetet.
    Zajac, Katarzyna
    University of Bayreuth, Germany .
    Palsgaard Andersen, Jacob
    Aage V Jensen Naturfond, Denmark .
    Andersson, Arne
    Jönköping County Administrative Board (Länsstyrelsen, Sweden .
    Börjesson, Gunnar
    Swedish University of Agriculture Science, Uppsala, Sweden .
    Bragazza, Luca
    University of Ferrara, Italy .
    Dise, Nancy B.
    Manchester Metropolitan University, UK.
    Keuskamp, Joost A.
    University of Utrecht, Netherlands .
    Larsson, Madeleine
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Lindgren, Per-Eric
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för mikrobiologi och molekylär medicin. Linköpings universitet, Hälsouniversitetet.
    Mattiasson, Peter
    Jönköping County Administrative Board (Länsstyrelsen, Sweden .
    Salomonsson, Jane
    Linköpings universitet, Institutionen för tema. Linköpings universitet, Filosofiska fakulteten.
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Verhoeyen, Jos T. A.
    University of Utrecht, Netherlands .
    Microclimatological consequences for plant and microbial composition in Sphagnum-dominated peatlands2014Ingår i: Boreal environment research, ISSN 1239-6095, E-ISSN 1797-2469, Vol. 19, nr 3, s. 195-208Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In three Scandinavian peatlands we studied to what extent plant and microbial community compositions are governed by local-scale microhabitat, with a special interest in the effect of aspect (i.e. exposition of slopes). Despite differences in solar irradiance between the south- and north-facing slopes, maximum temperature was elevated in the south-facing slopes at the most northern site only. Pore-water nutrient concentrations were not affected by aspect, yet dissolved organic carbon concentrations were higher in the south-facing microhabitats. This was likely caused by higher vascular plant biomass. Plant and microbial community composition clearly differed among sites. In all three sites, microhabitat (i.e. prevailing water-table depth) affected the plant and microbial community compositions. Aspect, however, did not affect community composition, even though microclimate significantly differed between the south- and the north-facing aspects at the northernmost site. Our results highlight the complex link between plant community composition, microbial community and environmental conditions, which deserves much more attention than currently in order to fully understand the effects of climate change on peatland ecosystem function.

  • 6.
    Sundberg, Carina
    et al.
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Al-Soud, Waleed A.
    University of Copenhagen, Denmark .
    Larsson, Madeleine
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Alm, Erik
    SMI, Sweden .
    Shakeri Yekta, Sepehr
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Sorensen, Soren J.
    University of Copenhagen, Denmark .
    Karlsson, Anna
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters2013Ingår i: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 85, nr 3, s. 612-626Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The microbial community of 21 full-scale biogas reactors was examined using 454 pyrosequencing of 16S rRNA gene sequences. These reactors included seven (six mesophilic and one thermophilic) digesting sewage sludge (SS) and 14 (ten mesophilic and four thermophilic) codigesting (CD) various combinations of wastes from slaughterhouses, restaurants, households, etc. The pyrosequencing generated more than 160 000 sequences representing 11 phyla, 23 classes, and 95 genera of Bacteria and Archaea. The bacterial community was always both more abundant and more diverse than the archaeal community. At the phylum level, the foremost populations in the SS reactors included Actinobacteria, Proteobacteria, Chloroflexi, Spirochetes, and Euryarchaeota, while Firmicutes was the most prevalent in the CD reactors. The main bacterial class in all reactors was Clostridia. Acetoclastic methanogens were detected in the SS, but not in the CD reactors. Their absence suggests that methane formation from acetate takes place mainly via syntrophic acetate oxidation in the CD reactors. A principal component analysis of the communities at genus level revealed three clusters: SS reactors, mesophilic CD reactors (including one thermophilic CD and one SS), and thermophilic CD reactors. Thus, the microbial composition was mainly governed by the substrate differences and the process temperature.

  • 7.
    Gustavsson, Jenny
    et al.
    Linköpings universitet, Institutionen för tema. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Shakeri Yekta, Sepehr
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Karlsson, Anna
    Linköpings universitet, Biogas Research Center. Scandinavian Biogas Fuels AB, Sweden .
    Ejlertsson, Jörgen
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Skyllberg, Ulf
    Linköpings universitet, Biogas Research Center. Swedish University of Agriculture Science, Sweden .
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten. Linköpings universitet, Biogas Research Center.
    Bioavailability of cobalt and nickel during anaerobic digestion of sulfur-rich stillage for biogas formation2013Ingår i: Applied Energy, ISSN 0306-2619, E-ISSN 1872-9118, Vol. 112, s. 473-477Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Addition of Co and Ni often improves the production of biogas during digestion of organic matter, i.e. increasing CH4-production, process stability and substrate utilization which often opens for higher organic loading rates (OLRs). The effect of Co and Ni addition was evaluated by measuring methane production, volatile solids reduction, pH and concentration of volatile fatty acids (VFAs). A series of six lab-scale semi-continuously fed biogas tank reactors were used for this purpose. The chemical forms and potential bioavailability of Co and Ni were examined by sequential extraction, acid volatile sulfide extraction (AVS) and simultaneously extracted metals. Furthermore, the sulfur speciation in solid phase was examined by sulfur X-ray absorption near edge structure spectroscopy. The effect of Co and Ni deficiency on the microbial community composition was analyzed using quantitative polymerase chain reaction and 454-pyrosequencing. The results showed that amendment with Co and Ni was necessary to maintain biogas process stability and resulted in increased CH4-production and substrate utilization efficiency. 10-20% of the total Co concentration was in dissolved form and should be regarded as easily accessible by the microorganisms. In contrast, Ni was entirely associated with organic matter/sulfides (mainly AVS) and regarded as very difficult to take up. Still Ni had stimulatory effects suggesting mechanisms such as dissolution of NiS to be involved in the regulation of Ni availability for the microorganisms. The microbial community structure varied in relation to the occurrence of Ni and Co. The acetate-utilizing Methanosarcinales dominated during stable process performance, i.e. when both Co and Ni were supplied, while hydrogenotrophic Methanomicrobiales increased together with VFA concentrations under Co or Ni deficiency. The increase was more pronounced at Co limitation. This study demonstrates that there are good possibilities to improve the performance of biogas processes digesting sulfur-rich substrates by supplementation of Co and Ni.

  • 8.
    Karlsson, Anna
    et al.
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Einarsson, Peter
    Linköpings universitet, Institutionen för tema. Linköpings universitet, Filosofiska fakulteten.
    Schnurer, Anna
    Swedish University of Agriculture Science, Sweden .
    Sundberg, Carina
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Filosofiska fakulteten.
    Ejlertsson, Jörgen
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Impact of trace element addition on degradation efficiency of volatile fatty acids, oleic acid and phenyl acetate and on microbial populations in a biogas digester2012Ingår i: Journal of Bioscience and Bioengineering, ISSN 1389-1723, E-ISSN 1347-4421, Vol. 114, nr 4, s. 446-452Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    The effect of trace element addition on anaerobic digestion of food industry- and household waste was studied using two semi-continuous lab-scale reactors, one (R30+) was supplied with Fe, Co and Ni, while the other (R30) acted as a control. Tracer analysis illustrated that methane production from acetate proceeded through syntrophic acetate oxidation (SAO) in both digesters. The effect of the trace elements was also evaluated in batch assays to determine the capacity of the microorganisms of the two digesters to degrade acetate, phenyl acetate, oleic acid or propionate, butyrate and valerate provided as a cocktail. The trace elements addition improved the performance of the process giving higher methane yields during start-up and early operation and lower levels of mainly acetate and propionate in the R30+ reactor. The batch assay showed that material from R30+ gave effects on methane production from all substrates tested. Phenyl acetate was observed to inhibit methane formation in the R30 but not in the R30+ assay. A real-time PCR analysis targeting methanogens on the order level as well as three SAO bacteria showed an increase in Methanosarcinales in the R30+ reactor over time, even though SAO continuously was the dominating pathway for methane production. Possibly, this increase explains the low VFA-levels and higher degradation rates observed in the R30+ batch incubations. These results show that the added trace elements affected the ability of the microflora to degrade VFAs as well as oleic acid and phenyl acetate in a community, where acetate utilization is dominated by SAO.

  • 9.
    Sundberg, Carina
    et al.
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Sundblad-Tonderski, Karin
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Lindgren, Per-Eric
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Ammonia oxidation and the corresponding bacterial communities in two overland flow areas treating landfill leachate or wastewater2011Ingår i: Overland Flow and Surface Runoff / [ed] Tommy S W Wong, Nova Science Publishers, Inc., 2011, s. 346-Kapitel i bok, del av antologi (Refereegranskat)
    Abstract [en]

    A high diversity of ammonium oxidising bacteria (AOB) has been observed in overland flow areas (OFA) treating ammonia-rich landfill leachate. The current section aimed to explore if treatment OFAs in general supports more diverse AOB communities than conventional treatment systems, or if it is a result of effluent composition. The potential ammonium oxidation and the AOB community composition were studied during three seasons in an OFA where one part received wastewater and the other landfill leachate. The AOB communities were investigated using group-specific PCR primers targeting the 16S rRNA gene, and analysed by DGGE and nucleotide sequencing. The potential ammonia oxidation, studied by short-time slurry incubation, was higher in the landfill OFA than in the wastewater area and highest in the litter layer. Higher activity correlated with the appearance of Nitrosomonas sp. belonging to cluster 7. Both overland flow areas supported a more diverse AOB community than in common wastewater treatment plants. Fifteen different AOB sequences were detected, but only three were observed in both OFAs, pointing to the impact of the effluent quality and/or the hydraulic load. The wastewater OFA, which received a higher load of effluents with 5-10 times lower ammonia concentrations, was dominated by AOB populations that are usually found in less favourable conditions.

  • 10. Hellman, Jan
    et al.
    Ek, Anders
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Johansson, Mariana
    Svensson, Bo
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Karlsson, Martin
    Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär Bioteknik. Linköpings universitet, Tekniska högskolan.
    Mechanisms of increased methane production through re-circulation of magnetic biomass carriers in an experimental continuously stirred tank reactor2010Konferensbidrag (Refereegranskat)
    Abstract [en]

    Magnetite particles were used in a semi-continuous process as magnetic biomass carriers to separate and re-introduce microorganisms in a CSTR reactor. In comparison to a control reactor the methane content during the semi-continuous process was elevated when magnetite particles were used. The difference was most apparent during the fermentative step directly after feeding and upon direct spiking with volatile fatty acids. Total DNA quantification of the separated magnetite particles revealed high association of microorganisms. Furthermore, quantitative real-time PCR analysis of the associated microbial consortia indicated that the hydrogenotrophic Methanobacteriales was overrepresented at the particle surface. Thus, the increased methane production could be coupled to both the crowding and shorter interspecies distances between the groups involved in anaerobic digestion, as well as a preferential adsorption of hydrogenotrophs. By bringing the hydrogenotrophs closer to the primary fermentative bacteria and increasing their relative number the produced hydrogen during acidogenesis is more effectively utilized and more carbon dioxide is converted to methane. Furthermore, by the same cause, the rate of acetogenesis increased as the hydrogenotrophs more effectively could consume the hydrogen produced and thereby keep the hydrogen partial pressure low.

  • 11.
    Sundberg, Carina
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Sundblad-Tonderski, Karin
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Lindgren, Per-Eric
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Development of the community structure and activity of ammoniaoxidising bacteria in overland flow systems used to treat landfill leachates2009Ingår i: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672Artikel i tidskrift (Övrigt vetenskapligt)
    Abstract [en]

    Ammonium in landfill leachates can be nitrified in overland flow areas (OFA). We studied OFAs to investigate if changes occur in the ammonium oxidizing community as the ecosystem develops, and the influence of different operating conditions. Samples were collected from the macrophyte litter layers, the rhizospheres and the sediments in their associated settling ponds in May, August, and November during four years. Potential ammonia oxidation (PAO) was investigated by a short-term slurry incubation method. The composition of the ammonia-oxidising bacteria (AOB) communities was investigated by PCR, using a group-specific primer pair, followed by denaturing gradient gel electrophoresis and subsequent sequencing.

    A shift from a Nitrosomonas community to a mixture of Nitrosomonas and Nitrosospira was and a gradual increase in PAO was observed, but only in the litter layer in the youngest OFA. Both OFAs had diverse AOB communities belonging to six different clusters. Nitrosomonas clusters predominated in the OFA with higher PAO, whereas Nitrosospira clusters were more common in the OFA with lower PAO. There was a seasonal increase of AOB populations in the OFA that was not in use during winter, and a more stable composition of the AOB community and the PAO in the OFA with year-round application. Keywords: Ammonia-oxidising bacterial community; Landfill leachates; Nitrification; Overland flow; 16S rDNA; Denaturing gradient gel electrophoresis.

  • 12.
    Faulwetter, Jennifer L
    et al.
    Montana State University.
    Gagnon, Vincent
    University of Montreal.
    Sundberg, Carina
    Linköpings universitet, Institutionen för tema, Tema vatten i natur och samhälle. Linköpings universitet, Filosofiska fakulteten.
    Chazarenc, Florent
    Ecole Mines Nantes.
    Burr, Mark D
    Montana State University.
    Brisson, Jacques
    University of Montreal.
    Camper, Anne K
    Montana State University.
    Stein, Otto R
    Montana State University.
    Microbial processes influencing performance of treatment wetlands: A review2009Ingår i: ECOLOGICAL ENGINEERING, ISSN 0925-8574, Vol. 35, nr 6, s. 987-1004Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    This review summarizes the microbial mechanisms responsible for removal of carbon, nitrogen. and sulfur compounds in treatment wetlands (TWs) and identifies, categorizes and compares various techniques, from plate count to more modern genomic methods used to elucidate these mechanisms. Removal of a particular pollutant is typically associated with a specific microbial functional group, therefore employment of design and operational methodologies that enhance the activity of that group will better optimize performance. Redox condition is a manipulable parameter that can be used to optimize growth of a targeted functional group, therefore factors influencing the TW redox condition and its influence on organic carbon removal mechanisms are emphasized. Environmental factors influencing growth and activity of N and S cycling microbes (including temperature, pH, salinity, plant species selection and availability of organic carbon and/or inhibiting substances) are discussed with particular attention to factors that might be manipulated. This information is used to offer design and operational methodologies that might enhance growth of a desirable microbial functional group and project what additional microbially-focused research is required to better optimize TW performance.

  • 13.
    Sundberg, Carina
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Nitrifyers in constructed wetlands treating landfill leachates2008Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
    Abstract [en]

    Landfill leachate is produced many years after a landfill site closes. Hence, treatment by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full-scale treatment systems are influenced by climatic variables that affect the microbial community. Also, the operational strategy can have a considerable impact on both activity and composition of the microorganisms. Many studies have measured inflow/outflow water quality in treatment systems. Such “black box” studies describe the treatment efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes.

    In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the corresponding bacterial communities in constructed wetlands treating landfill leachates. Variations in the potential activity in full-scale systems were investigated over several years, using short-term incubation. The composition of the bacterial communities was investigated using a group-specific PCR primer pair targeting the 16S rRNA genes or a primer pair targeting the funtional gene nosZ. The PCR products were analysed by denaturing gradient gelelectrophoresis and subsequent nucleotide sequencing and phylogentic analysis.

    A stable ammonia-oxidising bacterial (AOB) community composition and potential ammonia-oxidation (PAO) were detected in the system with a year-round operation. On the other hand, changes in the AOB community composition which followed the operational schedule were detected in the overland flow area (OFA) running seasonally. Furthermore, the influence of operational strategy was indicated by a low PAO in the wastewater overland flow area and compact constructed wetland receiving high hydraulic loads, indicating the value of aeration. Higher PAO was detected in the OFAs where the hydraulic load followed literature guidelines.

    All systems supported diverse AOB communities, represented by several Nitrosomonas and Nitrosospira populations. The number of different populations detected in these wetlands was much higher than reported in municipal wastewater treatment plants, and differed from those in a parallel OFA treating municipal wastewater. Furthermore, the large variation in both potential activity and sequences detected in replicate samples suggests that such systems are spatially heterogenic.

    Delarbeten
    1. Overland flow systems for treatment of landfill leachates: Potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season
    Öppna denna publikation i ny flik eller fönster >>Overland flow systems for treatment of landfill leachates: Potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season
    2007 (Engelska)Ingår i: Soil Biology and Biochemistry, ISSN 0038-0717, E-ISSN 1879-3428, Vol. 39, nr 1, s. 127-138Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Overland flow systems are useful for treating landfill leachates, because they provide favourable conditions for nitrification and they are easy to maintain. However, little is known about the microbial communities in such systems or the nitrification capacity of those microorganisms. In this study, seasonal variations in potential nitrification and in community composition of nitrifying bacteria were investigated in two overland flow areas receiving leachate from landfills at Korslöt and Hagby, Sweden. Samples were collected in the settling ponds sediment and at two depths in the overland flow areas (the macrophyte litter layer and the rhizosphere) in May, August and November 2003. A short-term incubation method was used to measure potential oxidation of ammonia and nitrite (designated PAO and PNO). The ammonia-oxidising bacterial (AOB) community was investigated using a 16S rRNA gene approach that included PCR amplification and analysis of PCR products by denaturing gradient gel electrophoresis (DGGE), followed by nucleotide sequencing and phylogenetic analysis.

    PAO was determined in the range 5–2700 (NO2−+NO3−)-N g−1 dw d−1 and PNO in the range 60–2000 μg NO2−-N g−1 dw d−1. At Korslöt, PAO and PNO showed similar temporal variation in the different ecosystems, whereas no such relationship was noticed at Hagby. Considering both sites, there was no obvious change in the composition of the AOB community over the growing season. However, the composition did differ between the ecosystems: Nitrosomonas-like sequences were more common in the ponds, and in the litter layers they were found as often as Nitrosospira-like sequences, whereas Nitrosospira-like sequences were more common in the rhizospheres. Altogether, we found nine different AOB sequences, five Nitrosomonas-like and four Nitrosospira-like, which belonged to clusters 0, 2, 3b, 6a, 6b and 7. There was no apparent relationship between the number of AOB populations and the PAO in different soil layers and sediments.

    Ort, förlag, år, upplaga, sidor
    Elesevire, 2007
    Nyckelord
    Ammonia-oxidising bacterial community; Landfill leachates; Nitrification; Overland flow; 16S rDNA; Denaturing gradient gel electrophoresis
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:liu:diva-17919 (URN)10.1016/j.soilbio.2006.06.016 (DOI)
    Tillgänglig från: 2009-04-24 Skapad: 2009-04-24 Senast uppdaterad: 2018-03-13Bibliografiskt granskad
    2. Potential nitrification and denitrification and the corresponding composition of the bacterial communities in a compact constructed wetland treating landfill leachates
    Öppna denna publikation i ny flik eller fönster >>Potential nitrification and denitrification and the corresponding composition of the bacterial communities in a compact constructed wetland treating landfill leachates
    2007 (Engelska)Ingår i: Water Science and Technology, ISSN 0273-1223, E-ISSN 1996-9732, Vol. 56, nr 3, s. 159-166Artikel i tidskrift (Refereegranskat) Published
    Abstract [en]

    Constructed wetlands can be used to decrease the high ammonium concentrations in landfill leachates. We investigated nitrification/denitrification activity and the corresponding bacterial communities in landfill leachate that was treated in a compact constructed wetland, Tveta Recycling Facility, Sweden. Samples were collected at three depths in a filter bed and the sediment from a connected open pond in July, September and November 2004. Potential ammonia oxidation was measured by short-term incubation method and potential denitrification by the acetylene inhibition technique. The ammonia-oxidising and the denitrifying bacterial communities were investigated using group-specific PCR primers targeting 16S rRNA genes and the functional gene nosZ, respectively. PCR products were analysed by denaturing gradient gel electrophoresis and nucleotide sequencing. The same degree of nitrification activity was observed in the pond sediment and at all levels in the filter bed, whereas the denitrification activity decreased with filter bed depth. Denitrification rates were higher in the open pond, even though the denitrifying bacterial community was more diverse in the filter bed. The ammonia-oxidising community was also more varied in the filter bed. In the filter bed and the open pond, there was no obvious relationship between the nitrification/denitrification activities and the composition of the corresponding bacterial communities.

    Nyckelord
    Denitrification, DGGE, landfill leachates, nitrification; nosZ, 16S rrNA
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:liu:diva-17920 (URN)10.2166/wst.2007.524 (DOI)17802851 (PubMedID)
    Tillgänglig från: 2009-04-24 Skapad: 2009-04-24 Senast uppdaterad: 2018-03-13Bibliografiskt granskad
    3. Development of the community structure and activity of ammoniaoxidising bacteria in overland flow systems used to treat landfill leachates
    Öppna denna publikation i ny flik eller fönster >>Development of the community structure and activity of ammoniaoxidising bacteria in overland flow systems used to treat landfill leachates
    2009 (Engelska)Ingår i: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672Artikel i tidskrift (Övrigt vetenskapligt) Submitted
    Abstract [en]

    Ammonium in landfill leachates can be nitrified in overland flow areas (OFA). We studied OFAs to investigate if changes occur in the ammonium oxidizing community as the ecosystem develops, and the influence of different operating conditions. Samples were collected from the macrophyte litter layers, the rhizospheres and the sediments in their associated settling ponds in May, August, and November during four years. Potential ammonia oxidation (PAO) was investigated by a short-term slurry incubation method. The composition of the ammonia-oxidising bacteria (AOB) communities was investigated by PCR, using a group-specific primer pair, followed by denaturing gradient gel electrophoresis and subsequent sequencing.

    A shift from a Nitrosomonas community to a mixture of Nitrosomonas and Nitrosospira was and a gradual increase in PAO was observed, but only in the litter layer in the youngest OFA. Both OFAs had diverse AOB communities belonging to six different clusters. Nitrosomonas clusters predominated in the OFA with higher PAO, whereas Nitrosospira clusters were more common in the OFA with lower PAO. There was a seasonal increase of AOB populations in the OFA that was not in use during winter, and a more stable composition of the AOB community and the PAO in the OFA with year-round application. Keywords: Ammonia-oxidising bacterial community; Landfill leachates; Nitrification; Overland flow; 16S rDNA; Denaturing gradient gel electrophoresis.

    Nyckelord
    Ammonia-oxidising bacterial community, Landfill leachates, Nitrification, Overland flow, 16S rDNA, Denaturing gradient gel electrophoresis
    Nationell ämneskategori
    Medicin och hälsovetenskap
    Identifikatorer
    urn:nbn:se:liu:diva-17921 (URN)
    Tillgänglig från: 2009-04-24 Skapad: 2009-04-24 Senast uppdaterad: 2018-03-13Bibliografiskt granskad
    4. Ammonia oxidation and the corresponding bacterial communities in two overland flow areas treating landfill leachate or wastewater
    Öppna denna publikation i ny flik eller fönster >>Ammonia oxidation and the corresponding bacterial communities in two overland flow areas treating landfill leachate or wastewater
    2011 (Engelska)Ingår i: Overland Flow and Surface Runoff / [ed] Tommy S W Wong, Nova Science Publishers, Inc., 2011, s. 346-Kapitel i bok, del av antologi (Refereegranskat)
    Abstract [en]

    A high diversity of ammonium oxidising bacteria (AOB) has been observed in overland flow areas (OFA) treating ammonia-rich landfill leachate. The current section aimed to explore if treatment OFAs in general supports more diverse AOB communities than conventional treatment systems, or if it is a result of effluent composition. The potential ammonium oxidation and the AOB community composition were studied during three seasons in an OFA where one part received wastewater and the other landfill leachate. The AOB communities were investigated using group-specific PCR primers targeting the 16S rRNA gene, and analysed by DGGE and nucleotide sequencing. The potential ammonia oxidation, studied by short-time slurry incubation, was higher in the landfill OFA than in the wastewater area and highest in the litter layer. Higher activity correlated with the appearance of Nitrosomonas sp. belonging to cluster 7. Both overland flow areas supported a more diverse AOB community than in common wastewater treatment plants. Fifteen different AOB sequences were detected, but only three were observed in both OFAs, pointing to the impact of the effluent quality and/or the hydraulic load. The wastewater OFA, which received a higher load of effluents with 5-10 times lower ammonia concentrations, was dominated by AOB populations that are usually found in less favourable conditions.

    Ort, förlag, år, upplaga, sidor
    Nova Science Publishers, Inc., 2011
    Nyckelord
    Runoff -- Mathematical models, Streamflow -- Mathematical models, Frictional resistance (Hydrodynamics), ´Water Pollution, Nitrifying bacteria, SCIENCE / Earth Sciences / Hydrology, SCIENCE / Earth Sciences / Hydrology
    Nationell ämneskategori
    Naturvetenskap
    Identifikatorer
    urn:nbn:se:liu:diva-17922 (URN)978-1-61122-868-7 (ISBN)
    Tillgänglig från: 2009-04-24 Skapad: 2009-04-24 Senast uppdaterad: 2018-03-13Bibliografiskt granskad
  • 14.
    Sundberg, Carina
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Stendahl, Jenny S. K.
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Sundblad-Tonderski, Karin
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Lindgren, Per-Eric
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Overland flow systems for treatment of landfill leachates: Potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season2007Ingår i: Soil Biology and Biochemistry, ISSN 0038-0717, E-ISSN 1879-3428, Vol. 39, nr 1, s. 127-138Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Overland flow systems are useful for treating landfill leachates, because they provide favourable conditions for nitrification and they are easy to maintain. However, little is known about the microbial communities in such systems or the nitrification capacity of those microorganisms. In this study, seasonal variations in potential nitrification and in community composition of nitrifying bacteria were investigated in two overland flow areas receiving leachate from landfills at Korslöt and Hagby, Sweden. Samples were collected in the settling ponds sediment and at two depths in the overland flow areas (the macrophyte litter layer and the rhizosphere) in May, August and November 2003. A short-term incubation method was used to measure potential oxidation of ammonia and nitrite (designated PAO and PNO). The ammonia-oxidising bacterial (AOB) community was investigated using a 16S rRNA gene approach that included PCR amplification and analysis of PCR products by denaturing gradient gel electrophoresis (DGGE), followed by nucleotide sequencing and phylogenetic analysis.

    PAO was determined in the range 5–2700 (NO2−+NO3−)-N g−1 dw d−1 and PNO in the range 60–2000 μg NO2−-N g−1 dw d−1. At Korslöt, PAO and PNO showed similar temporal variation in the different ecosystems, whereas no such relationship was noticed at Hagby. Considering both sites, there was no obvious change in the composition of the AOB community over the growing season. However, the composition did differ between the ecosystems: Nitrosomonas-like sequences were more common in the ponds, and in the litter layers they were found as often as Nitrosospira-like sequences, whereas Nitrosospira-like sequences were more common in the rhizospheres. Altogether, we found nine different AOB sequences, five Nitrosomonas-like and four Nitrosospira-like, which belonged to clusters 0, 2, 3b, 6a, 6b and 7. There was no apparent relationship between the number of AOB populations and the PAO in different soil layers and sediments.

  • 15.
    Sundberg, Carina
    et al.
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Sundblad-Tonderski, Karin
    Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
    Lindgren, Per-Erik
    Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk mikrobiologi. Linköpings universitet, Hälsouniversitetet.
    Potential nitrification and denitrification and the corresponding composition of the bacterial communities in a compact constructed wetland treating landfill leachates2007Ingår i: Water Science and Technology, ISSN 0273-1223, E-ISSN 1996-9732, Vol. 56, nr 3, s. 159-166Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    Constructed wetlands can be used to decrease the high ammonium concentrations in landfill leachates. We investigated nitrification/denitrification activity and the corresponding bacterial communities in landfill leachate that was treated in a compact constructed wetland, Tveta Recycling Facility, Sweden. Samples were collected at three depths in a filter bed and the sediment from a connected open pond in July, September and November 2004. Potential ammonia oxidation was measured by short-term incubation method and potential denitrification by the acetylene inhibition technique. The ammonia-oxidising and the denitrifying bacterial communities were investigated using group-specific PCR primers targeting 16S rRNA genes and the functional gene nosZ, respectively. PCR products were analysed by denaturing gradient gel electrophoresis and nucleotide sequencing. The same degree of nitrification activity was observed in the pond sediment and at all levels in the filter bed, whereas the denitrification activity decreased with filter bed depth. Denitrification rates were higher in the open pond, even though the denitrifying bacterial community was more diverse in the filter bed. The ammonia-oxidising community was also more varied in the filter bed. In the filter bed and the open pond, there was no obvious relationship between the nitrification/denitrification activities and the composition of the corresponding bacterial communities.

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