liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
University of Michigan, USA.
Hebrew University of Jerusalem, Israel.
Catholic University of Louvain, Belgium.
Creighton University, USA.
Vise andre og tillknytning
2008 (engelsk)Inngår i: Autophagy, ISSN 1554-8627, E-ISSN 1554-8635, Vol. 4, nr 2, s. 151-175Artikkel, forskningsoversikt (Fagfellevurdert) Published
Abstract [en]

Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

sted, utgiver, år, opplag, sider
Landes Bioscience , 2008. Vol. 4, nr 2, s. 151-175
Emneord [en]
autolysosome; autophagosome; flux; lysosome; phagophore; stress; vacuole
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-92743ISI: 000253008800003PubMedID: 18188003OAI: oai:DiVA.org:liu-92743DiVA, id: diva2:622049
Tilgjengelig fra: 2013-05-20 Laget: 2013-05-20 Sist oppdatert: 2017-12-06bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

PubMed

Person

Brunk, Ulf T.Terman, Alexei

Søk i DiVA

Av forfatter/redaktør
Brunk, Ulf T.Terman, Alexei
Av organisasjonen
I samme tidsskrift
Autophagy

Søk utenfor DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric

pubmed
urn-nbn
Totalt: 1753 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf