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Generalized Pareto Distributions, Image Statistics and Autofocusing in Automated Microscopy
Linköpings universitet, Institutionen för teknik och naturvetenskap, Medie- och Informationsteknik. Linköpings universitet, Tekniska fakulteten. Linköpings universitet, Centrum för medicinsk bildvetenskap och visualisering, CMIV.ORCID-id: 0000-0001-7557-4904
2015 (engelsk)Inngår i: GEOMETRIC SCIENCE OF INFORMATION, GSI 2015, Springer-Verlag New York, 2015, s. 96-103Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

We introduce the generalized Pareto distributions as a statistical model to describe thresholded edge-magnitude image filter results. Compared to the more common Weibull or generalized extreme value distributions these distributions have at least two important advantages, the usage of the high threshold value assures that only the most important edge points enter the statistical analysis and the estimation is computationally more efficient since a much smaller number of data points have to be processed. The generalized Pareto distributions with a common threshold zero form a two-dimensional Riemann manifold with the metric given by the Fisher information matrix. We compute the Fisher matrix for shape parameters greater than -0.5 and show that the determinant of its inverse is a product of a polynomial in the shape parameter and the squared scale parameter. We apply this result by using the determinant as a sharpness function in an autofocus algorithm. We test the method on a large database of microscopy images with given ground truth focus results. We found that for a vast majority of the focus sequences the results are in the correct focal range. Cases where the algorithm fails are specimen with too few objects and sequences where contributions from different layers result in a multi-modal sharpness curve. Using the geometry of the manifold of generalized Pareto distributions more efficient autofocus algorithms can be constructed but these optimizations are not included here.

sted, utgiver, år, opplag, sider
Springer-Verlag New York, 2015. s. 96-103
Serie
Lecture Notes in Computer Science, ISSN 0302-9743, E-ISSN 1611-3349 ; 9389
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-127703DOI: 10.1007/978-3-319-25040-3_11ISI: 000374288700011ISBN: 978-3-319-25039-7 (tryckt)ISBN: 978-3-319-25040-3 (tryckt)OAI: oai:DiVA.org:liu-127703DiVA, id: diva2:926687
Konferanse
2nd International SEE Conference on Geometric Science of Information (GSI)
Forskningsfinansiär
Swedish Research Council, 2014-6227Swedish Foundation for Strategic Research , IIS11-0081Tilgjengelig fra: 2016-05-09 Laget: 2016-05-09 Sist oppdatert: 2025-02-07

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