Thermal imagery provides new opportunities to study concepts and processes in biology. Examples include using infrared (IR) cameras in educational activities to explore energy transfer and transformation in human physiology, animal thermoregulation, and plant metabolism. The user-friendly and visually intuitive nature of IR technology is well suited to the study of rapidly changing temperatures on biological surfaces, due to such energy transfers. IR cameras are therefore potentially helpful pedagogical tools for approaching the Energy and Matter crosscutting concept in the Life Sciences discipline of the Next Generation Science Standards.