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CXCL13 in laboratory diagnosis of Lyme neuroborreliosis-the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
Linköping University, Faculty of Medicine and Health Sciences. Linköping University, Department of Biomedical and Clinical Sciences, Division of Cell Biology. Reg Jonkoping Cty, Dept Lab Med Jonkoping.
Linköping University, Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection. Linköping University, Faculty of Medicine and Health Sciences. Reg Jonkoping Cty, Dept Lab Med Jonkoping.
Linköping University, Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection. Linköping University, Faculty of Medicine and Health Sciences. Reg Jonkoping Cty, Dept Infect Dis.
Linköping University, Department of Biomedical and Clinical Sciences. Linköping University, Faculty of Medicine and Health Sciences. Reg Jonkoping Cty, Dept Lab Med Jonkoping.
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2022 (English)In: European Journal of Clinical Microbiology and Infectious Diseases, ISSN 0934-9723, E-ISSN 1435-4373, Vol. 41, no 1, p. 175-179Article in journal (Refereed) Published
Abstract [en]

The chemokine CXCL13 is used as complement to serology in the diagnostics of Lyme neuroborreliosis (LNB). We evaluated and compared the semi-quantitative, cassette-based ReaScan CXCL13 assay with the quantitative recomBead CXCL13 assay using a collection of 209 cerebrospinal fluid samples. The categorical agreement between results interpreted as negative, grey zone, and positive by the two methods was 87%. The diagnostic sensitivity was higher using the recomBead assay, whereas specificity was higher using ReaScan. Few manual steps, and a short turn-around time with no batching of samples makes the ReaScan CXCL13 assay an attractive complement to serology in the diagnostics of LNB.

Place, publisher, year, edition, pages
Springer , 2022. Vol. 41, no 1, p. 175-179
Keywords [en]
Lyme neuroborreliosis; Diagnostics; CXCL13; ReaScan; recomBead
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-180499DOI: 10.1007/s10096-021-04350-yISI: 000705823200001PubMedID: 34626256OAI: oai:DiVA.org:liu-180499DiVA, id: diva2:1605671
Note

Funding Agencies|European Union through the European Regional Development FundEuropean Commission; Interreg North Sea Region Programme [J-No: 38-2-7-19]

Available from: 2021-10-25 Created: 2021-10-25 Last updated: 2023-05-04

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Haglund, Sofie

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Haglund, SofieLager, MalinGyllemark, PaulaAndersson, GärdaJonsson Henningsson, Anna
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Faculty of Medicine and Health SciencesDivision of Cell BiologyDivision of Inflammation and InfectionDepartment of Biomedical and Clinical SciencesDepartment of Clinical Microbiology
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European Journal of Clinical Microbiology and Infectious Diseases
Microbiology in the medical area

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