The aim of this thesis is to develop an electroactive in vitro model of the bone marrow(BM), termed "bone-on-a-chip," designed to replicate the BM microenvironment.Polypyrrole (PPy) doped with succinic acid (SUCA) was electropolymerized ontoa gold surface using a three-electrode setup. Chondrocyte-derived plasma membranenanofragments (PMNFs) of KUSA cells origin were covalently attached to the PPy(SUCA)surface via EDC/NHS coupling. The redox states of PPy were modulated from asfabricated (AF) to oxidized (OX) and reduced (RE) to mimic variations in bone density.The PPy surfaces were mineralized through the growth of minerals during a three-dayincubation in cell culture media. Characterization techniques assessed surface propertiesat each fabrication step. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measured electrochemical performance, while water contact angle (WCA)gauged hydrophobicity. SEM and energy-dispersive X-ray spectroscopy (EDX) evaluatedsurface morphology and mineral content post-mineralization. Further cell studies wereconducted on the mineralized PPy surfaces to assess their ability to support stem cell viability and remain in their undifferentiated state as stem cells without further differentiationinto other cell types. Mouse hematopoietic stem cells (HSCs) were subsequently isolated,sorted, and cultured on the surfaces.The RE state demonstrated the highest WCA and mineral content, followed by OXand AF. EDX confirmed significant differences in calcium (Ca) and phosphorus (P) levelsbetween surfaces with switched redox states (OX and RE) compared to AF and the negativecontrol. SEM images revealed clear differences seen in morphology between the redoxstates, although these differences appeared challenging to control. Preliminary cell studiessuggested that redox-modified surfaces might support undifferentiated HSCs better thanthe AF state and controls; however, reproducibility remains a challenge.This study highlights the potential of electroactive surfaces in creating a bone-on-achip model to better understand bone marrow dynamics and stem cell behavior, pavingthe way for applications in stem cell research and therapeutic advancements.