Decreasing sedentary behavior and taking active short duration breaks offers several positive benefits for office workers. One way to encourage active short duration breaks in an office environment is through the use of casual exergames. Previous research has shown that casual exergames offer benefits in terms of enjoyment, exertion, and increased positive affect. However, little is known about whether this holds true for short duration (e.g., 2 minutes) casual exergames and if seated compared to standing gameplay offers similar benefits. Through a quasi-experimental within-subjects design we investigated whether positive affect differs before and after short duration casual exergaming for seated and standing positions, and if seated compared to standing short duration casual exergaming differs in terms of positive affect, perceived exertion, enjoyment, and performance. The main results showed that short duration casual exergaming increased positive affect significantly for both standing and seated gameplay and that there were no differences between seated and standing gameplay in terms of positive affect, perceived exertion, enjoyment, and performance.