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Coagulation depth estimation using a line scanner for depth-resolved laser speckle contrast imaging
Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0003-4910-0291
Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0001-5912-3281
Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering.ORCID iD: 0000-0002-3497-1257
2024 (English)In: Biomedical Optics Express, E-ISSN 2156-7085, Vol. 15, no 8, p. 4486-4497Article in journal (Refereed) Published
Abstract [en]

Partial-thickness burn wounds extend partially through the dermis, leaving many pain receptors intact and making the injuries very painful. Due to the painfulness, quick assessment of the burn depth is important to not delay surgery of the wound if needed. Laser speckle imaging (LSI) of skin blood flow can be helpful in finding severe coagulation zones with impaired blood flow. However, LSI measurements are typically too superficial to properly reach the full depth of the adult dermis and cannot resolve the flow in depth. Diffuse correlation spectroscopy (DCS) uses varying source-detector separations to allow differentiation of flow depths but requires time-consuming 2D scanning to form an image of the burn area. We here present a prototype for a hybrid DCS and LSI technique called speckle contrast diffuse correlation spectroscopy (scDCS) with the novel approach of using a laser line as a source and using the speckle contrast of averaged images to obtain an estimate of static scattering in the tissue. This will allow for fast non-contact 1D scanning to perform 3D tomographic imaging, making quantitative estimates of the depth and area of the coagulation zone from burn wounds. Simulations and experimental results from a volumetric flow phantom and a gelatin wedge phantom show promise to determine coagulation depth. The aim is to develop a method that, in the future, could provide more quantitative estimates of coagulation depth in partial thickness burn wounds to better estimate when surgery is needed.

Place, publisher, year, edition, pages
Optica Publishing Group , 2024. Vol. 15, no 8, p. 4486-4497
National Category
Medical Imaging
Identifiers
URN: urn:nbn:se:liu:diva-207466DOI: 10.1364/BOE.529043ISI: 001302557200003OAI: oai:DiVA.org:liu-207466DiVA, id: diva2:1896468
Note

Funding Agencies|Knut and Alice Wallenberg Centre for Molecular Medicine; Linkoeping University Area of Strength CircM

Available from: 2024-09-10 Created: 2024-09-10 Last updated: 2025-02-09

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