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2021 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 195, article id 113823Article in journal (Refereed) Published
Abstract [en]
A highly sensitive and accurate electrospray liquid chromatography tandem-mass spectrometry (ESI-LC–MS/MS) method for determination of testosterone in human serum and saliva was developed and validated. Accurate quantification of testosterone in human matrices is essential in diagnosis and management of androgen status in men, women and children, and in forensic investigations of suspected abuse of anabolic androgenic steroids. Chromatography was performed on an HSS-T3 C18 column with a total run-time of 5.5 min. The tandem mass spectrometry was operated in positive electrospray ionization mode with multiple reaction monitoring. Serum and saliva samples of 200 μL, were prepared by solid-phase extraction using a 96-well plate following precipitation with 200 μL methanol. 13C labeled testosterone was used as internal standard for quantification. The standard curve was linear within the range of 4−1000 pg/mL and the limit of quantification of both serum and salivary testosterone was 4 pg/mL. Accuracy were 99–101 % and 93–95 % with between-run imprecision in serum and saliva, respectively, and inter- and intra-assay coefficients of variation were less than 9.2 %. The method proved to be applicable for determination of testosterone over a wide range of concentrations in serum and saliva samples from clinical patients with various androgen disorders, healthy male and female adults as well as from forensic cases.
Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
Testosterone, Serum, Saliva, LC–MS/MS
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-173145 (URN)10.1016/j.jpba.2020.113823 (DOI)000610841900007 ()113823 (Local ID)113823 (Archive number)113823 (OAI)
Note
Funding:Strategic Area in Forensic Science; Forskningsradet i Sydostra Sverige (FORSS) [713391]
2021-02-042021-02-042022-05-23Bibliographically approved